| Group | Club/Team Name | Team ID | Head Coach |
| A1 | All-In FC TN All-In FC TN 10B Chupacabras |
0443-01SLB4U-0006 | Aaron Alva |
| A2 | Armada Futbol Club Armada FC 2010 Boys Red |
0436-012B4U-0009 | Mirgen Bushati |
| A3 | AYSO Alliance Knoxville - 01 AYSOR124 ALLIANCE 10B |
0292-013B4U-0026 | Clement Hendrickson |
| A4 | Chattanooga FC Academy Chattanooga FC - 2010B Navy |
0316-022B4U-7928 | Derek Greene |
| A5 | Chattanooga Red Wolves Academy Chattanooga Red Wolves Academy - 2010B Red |
0324-022B4U-0004 | Owen Click |
| A6 | Chattanooga FC Academy CSM Forge U14 Boys |
0316-022B4U-7953 | Jose Ramirez |
| A7 | FC Alliance Select Travel Teams FC Alliance 2010B RL |
0204-012B4U-0263 | Kevin Cardona |
| A8 | Music City Soccer Club Music City FC 10B Premier |
0517-013B4U-3500 | Francisco Aleman |
| A9 | One Knoxville One Knox 10B- Orange |
0293-01D2B4U-0022 | William Comstock |
| A10 | Wilson United Soccer Club Wilson United SC 2010 B Black |
0406-012B4U-0003 | Joel Gibbs |