| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tennessee United Soccer Club TUSC 2010 Boys Red |
0424-012B4U-0017 | John Lehn |
| A2 | Tennessee Soccer Club TSC Williamson 2010 Boys Showcase |
0522-012B4U-2223 | Michael Alley |
| A3 | TN Tempo FC Academy TN Tempo FC Academy - 2010B |
0592-012B4U-0012 | Nathaniel Bedai |
| A4 | Stones River Futbol Club - 01 Stones River FC 2010 Red |
0521-013B4U-0012 | Joshua Stone |
| A5 | Nashville United Soccer Academy - 01 NUSA B10 Navy |
0534-012B4U-0021 | Julian Lucumi Villegas |
| A6 | Clarksville Soccer Club Clarksville SC - B2010 Maroon |
0422-012B4U-0011 | Alejandro Herrera |
| A7 | ALBION SC Nashville (Formerly Southern Premier Soccer) ALBION SC Nashville - B10 Academy |
0540-02D2B4U-0035 | Brent Goulet |
| A8 | Action Elite Soccer Club (formerly known as Action Indoor Sports Soccer Assn.) Action Elite 2010B |
0501-012B4U-0001 | Zachary Cannon |
| A9 | Lobos Rush (Collierville Soccer Association) 2010 Lobos Rush E64 |
0603-012B4U-0035 | Zayrion Harris |
| A10 | Columbia Soccer Association - 01 2010 Columbia Arsenal |
0504-012B4U-0004 | Rey Martinez |