| Group | Club/Team Name | Team ID | Head Coach |
| A2 | Nashville United Soccer Academy - 01 NUSA B12 Maroon |
0534-01AB2U-0047 | Calvin Da Cunha |
| A3 | Kings Hammer KHM 12B Premier I |
0515-01DB2U-0203 | Brian Grogan |
| A4 | FC Alliance ChallengeTeams FC Alliance 2012B Pre-ECNL |
0204-021B3U-0223 | Caleb Lucas |
| A5 | Chattanooga Red Wolves Academy Chattanooga Red Wolves Academy 2012 Boys |
0324-02SJB2U-0058 | Mahmoud Mohamed |
| A6 | Chattanooga FC Academy Chattanooga FC - 2012B Navy |
0316-02SJB2U-7921 | Dalton Healy |