| Group | Club/Team Name | Team ID | Head Coach |
| A1 | All-In FC TN All-In FC TN 12B Mercurial |
0443-01ACB2U-0019 | Manuel Fuentes |
| A2 | Chattanooga FC Academy Chattanooga FC - 2013B Navy |
0316-02SJB1U-7920 | Jordan Replogle |
| A4 | Chattanooga FC Academy CSM Forge U12 Boys A |
0316-029B2U-7956 | Brett Robinson |
| A5 | FC Alliance ChallengeTeams FC Alliance 2013B Pre-ECNL |
0204-02SB1U-0227 | Caleb Lucas |
| A6 | FC Alliance ChallengeTeams FC Alliance 2013B Pre-ECRL |
0204-02SB1U-0228 | Johnny Bracewell |
| A7 | Tri-Cities United Soccer Club Tri-Cities UnIted 2012 Boys Select |
0106-01CB2U-0015 | Hector Galicia Escobar |
| A8 | One Knoxville One Knox 13B- Yellow |
0293-01SJB1U-0041 | Gustavo Alvarez-Suchini |